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| Silva,A.M.; Pires,E.G.; Abrantes,E.F.; Ferreira,L.R.P.; Gazzinelli,R.T.; Reis,L.F.L.. |
| The inflammatory response elicited by various stimuli such as microbial products or cytokines is determined by differences in the pattern of cellular gene expression. We have used the differential display RT-PCR (DDRT-PCR) strategy to identify mRNAs that are differentially expressed in various murine cell types stimulated with pro-inflammatory cytokines, microbial products or anti-inflammatory drugs. Mouse embryonic fibroblasts (MEFs) were treated with IFNs, TNF, or sodium salicylate. Also, peritoneal macrophages from C3H/Hej mice were stimulated with T. cruzi-derived GPI-mucin and/or IFN-<FONT FACE="Symbol">g</FONT>. After DDRT-PCR, various cDNA fragments that were differentially represented on the sequencing gel were recovered, cloned and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DDRT-PCR; Gene expression; Inflammation; TNF; Interferon. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700008 |
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| Santana,Flávia A.; Nunes,Francis M.F.; Vieira,Carlos U.; Machado,Maria Alice M.S.; Kerr,Warwick E.; Silva Jr,Wilson A.; Bonetti,Ana Maria. |
| We have compared gene expression, using the Differential Display Reverse Transcriptase - Polymerase Chain Reaction (DDRT-PCR) technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, and in both Natural and Juvenile Hormone III-induced adult queen. Six, out of the nine ESTs described here, presented differentially expressed in the phases L1 or L2, or even in both of them, suggesting that key mechanisms to the development of Melipona scutellaris are regulated in these stages. The combination HT11G-AP05 revealed in L1 and L2 a product which matches to thioredoxin reductase protein domain in the Clostridium sporogenes, an important protein during cellular oxidoreduction processes. This study... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melipona scutellaris; Gene expression; DDRT-PCR. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100008 |
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| WILLADINO,LILIA; CAMARA,TEREZINHA RANGEL; RIBEIRO,MARTA BARBOSA; AMARAL,DANIEL OLIVEIRA JORDÃO DO; SUASSUNA,FLAVIA; SILVA,MÁRCIA VANUSA DA. |
| ABSTRACT In the northeastern region of Brazil, saline soils are constraints to banana production, becoming necessary to understand the mechanisms of salt tolerance. Two bananas genotypes, Tap Maeo, tolerant, and Berlin, sensitive, were subjected to treatment with 50 mol m-3 NaCl or without salt. This study evaluated the effects of salt on the following physiological aspects: leaf area, content and distribution of Na+, membrane integrity, proton AT Pase activity. Besides, a search for differentially expressed genes was performed using the Differential Display technique. Tap Maeo genotype showed the smallest reduction in leaf area, smaller accumulation of Na+ and malondialdehyde (MDA), and higher activity of proton AT Pase activity. Two sequences... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DDRT-PCR; Leaf area; Musa; Na+; Antioxidative system; SOS pathway. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000200202 |
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